Back
Dr. Prabodh Kumar Trivedi

Area of Specialization

  • Elucidation and engineering for secondary plant pathways for enhanced nutritional value and important therapeutic molecules
  • Environmental biotechnology for heavy metal stress tolerance and accumulation in plants
  • Molecular basis of fruit ripening
  • Chloroplast genome organization of Populus deltoides
    I have developed expertise and leadership while working in above mentioned research areas far more than twenty-five years. Several competitive grants as different R&D projects in these areas have been funded by various funding agencies including International agencies. I successfully co-ordinated CSIR-network project entitled "Genomics of Medicinal Plants and Agronomically Important Traits (PlaGen; BSC-107)" consisting of seven CSIR laboratories involving more than 50 scientists and large number of research students. Progress of this project was evaluated as “Excellent” by Sectorial Monitoring Committee (SMC) and recommendation was made to develop Mission Mode projects using leads from the project. I have successfully completed NMITLI project on Withania genomics as PI and submitted final technical report. In addition, I have developed collaborations with various groups within as well as outside country to carryout projects related to interdisciplinary science. Due to expertise developed in the area of pathway engineering of secondary plant product development, Department of Biotechnology sanctioned Centre of Excellent on “Secondary plant product pathway engineering for enhanced nutritional quality and yield”. In addition, due to leadership in plant science research in country, The Indian National Science Academy (INSA), National Academy of Agriculture Sciences (NAAS) and National Academy of Sciences, India (NASI) have elected me as Fellow of these academies as well as DBT has selected for TATA Innovation Awards-2017. Recently, in addition to several CSIR and externally funded projects, I am co-ordinating CSIR NCP project entitled “Genome-editing for enhanced yield and quality traits (GE-plant)”. I organized “4th International Plant Physiology Congress (IPPC-2018)” during 2nd-5th December, 2018 which was attended by approximately 700 delegates from fifteen countries.


  • Ph.D. - Dr. R M L Avadh University, Faizabad* (Chemistry**)
  • M.Sc. - Lucknow University (Biochemistry)
  • B.Sc. - Lucknow University (Physics, Chemistry, Mathematics)
  • CSIR-Central Institute of Medicinal and Aromatic Plants - Director (2020)
  • CSIR-National Botanical Research Institute, Lucknow - Senior Principal Scientist (2013-2020)
  • CSIR-National Botanical Research Institute, Lucknow - Principal Scientist (2008-2013)
  • CSIR-National Botanical Research Institute, Lucknow - Senior Scientist (2005-2008)
  • CSIR-National Botanical Research Institute, Lucknow - Scientist C (1999-2005)
  • University of Maryland, College Park, USA, - Post-Doctoral Fellow (March 2002 - April 2002)
  • CSIR-National Botanical Research Institute, Lucknow - Scientist B (1994 - 1999)
  • Elected as Honorary Member of the Plant Tissue Culture Association, India (PTCA, India)
  • J.C. Bose National Fellowship
  • Fellow of Indian National Academy Sciences (FNA)-2020 .
  • Council Member, National Academy of Sciences, India
  • TATA Innovation Fellowship-2017 by Department of Biotechnology, New Delhi .
  • Member, Board of Studies, Academy of Scientific and Innovative Research (AcSIR).
  • Centre of Excellence and Innovation in Biotechnology for “Secondary plant product pathway engineering for enhanced nutritional quality and yield” by Department of Biotechnology, New Delhi .
  • Fellow of National Academy of Agriculture Sciences, India (FNAAS)-2016.
  • Fellow of National Academy of Sciences, India (FNASc)-2014.
  • Fellow of International Society of Environment Botanist (FISEB)-2017.
  • CSIR-Technology Award-2015 in association with CSIR-CIMAP, CSIR-CDRI and CSIR-IICB as PI team CSIR-NBRI for Development of Withania somnifera Agro-technology.
  • Member, Membership Scrutiny Committee (MSC), National Academy of Sciences, India.
  • Member, Board of Studies, Academy of Scientific and Innovative Research (AcSIR)
  • Academic Editor, PLoS One
  • Editorial Board Member, Scientific Reports (Nature Publishing Group)
  • Editorial Board Member, Physiology and Molecular Biology of Plants (Springer)
  • Editorial Board Member, International Journal of Plant and Environment
  • Member of Advisory Committee for Biotechnology, CST, UP
  • Expert Member of Institutional Bio Safety committee (IBSC), Integral University, Lucknow
  • Expert Member of Institutional Bio Safety committee (IBSC), BBAU, Lucknow
  • Expert Member of Institutional Bio Safety committee (IBSC), CSIR-CIMAP, Lucknow
  • Nominated as Expert Member for Research Project Evaluation (Avishkar 2015) organized by Maharashtra State Inter-University Convention
  • Member of Research Doctoral Committee, Integral University, Lucknow
  • Life member, Clean and Green Environment Society (CGES)
  • Life member, Society of Plant Biochemistry and Biotechnology (India)
  • Life member, Society of Plant Physiology and Biochemistry (India)
  • Life member, Society of Biological Chemists (India)
  • Life member, International Society on Environmental Botanists (ISEB)
  • Life member, Indian society of Cell Biology (ISCB)
  • Life member, Alumni Association of Dept. of Biochemistry, Lucknow University
  • External Member, Doctoral Committee, SGPGI, Lucknow
  • External Member, Technical Committee, SGPGI, Lucknow
  • Recognized Ph.D. supervisor of following Universities:
    1. Academy of Scientific and Innovative Research (AcSIR)
    2. University of Lucknow, Lucknow (Biochemistry and Botany)
    3. Kumaun University, Nainital (Interdisciplinary Research)
    4. Banaras Hindu University (Biochemistry and Botany), Varanasi
    5. Dr. A.P.J. Abdul Kalam Technical University, Lucknow

Patents granted:

  • Pharmaceutical composition for the treatment of diminution of bone tissue (No: 2014291615; Application granted on 26.09.2019) Australia Trivedi R, Mishra PR, Singh D, Khedgikar V, Kushwaha P, Adhikari S, Choudhari D, Gautam J, Kumar A, Karvande A, Verma A, Sharma S, Trivedi PK, Sangwan N, Sangwan RS .
  • Pharmaceutical composition for the treatment of diminution of bone tissue (Number: 14759347.9; Application granted on 13.11.2019) Europe Trivedi R, Mishra PR, Singh D, Khedgikar V, Kushwaha P, Adhikari S, Choudhari D, Gautam J, Kumar A, Karvande A, Verma A, Sharma S, Trivedi PK, Sangwan N, Sangwan RS .
  • Pharmaceutical composition for the treatment of diminution of bone tissue (Nunber. US10596115B2. Application granted on 24.03.2020) United States of America Trivedi R, Mishra PR, Singh D, Khedgikar V, Kushwaha P, Adhikari S, Choudhari D, Gautam J, Kumar A, Karvande A, Verma A, Sharma S, Trivedi PK, Sangwan N, Sangwan RS .

List of patents applied for:

  • Proteasomal inhibitors useful for osteogenic activity and pharmaceutical composition, thereof [osteoheal] (2145/DEL 2013 dated 17.07.2013; NF No. 0158NF2013/IN) India Trivedi R, Mishra PR, Sangwan N, Trivedi PK, Singh D, Sangwan RS, Kushwaha P, Khedgikar K, Adhikari S, Choudhari D, Swarup J, Kumar A, Karvande A, Verma A, Sharma S .
  • Pharmaceutical composition for the treatment of diminution of bone tissue (Application number: PCT/IN2014/000475; Publication number: WO2015008301-A1) filling date July 16, 2014 Trivedi R, Mishra PR, Singh D, Khedgikar V, Kushwaha P, Adhikari S, Choudhari D, Gautam J, Kumar A, Karvande A, Verma A, Sharma S, Trivedi PK, Sangwan N, Sangwan RS .
  • Pharmaceutical composition for the treatment of diminution of bone tissue (NF No. 0158NF2013/CA; Application number: 2917921) filling date Jan 20, 2016; Canada Trivedi R, Mishra PR, Singh D, Khedgikar V, Kushwaha P, Adhikari S, Choudhari D, Gautam J, Kumar A, Karvande A, Verma A, Sharma S, Trivedi PK, Sangwan N, Sangwan RS .

Highlights of the contribution to the area of specialization

I have more than twenty-four years of research experience including two years of Post doc in USA in the area of Plant Genomics and Biotechnology. Recent highlights of my contribution in specialized areas are as follows:

Pathway elucidation and engineering for secondary plant products and metabolites

Main focus has been to study metabolic pathways and regulation of gene expression involved in biosynthesis of important molecules beneficial to human health and enhanced nutritional quality.
  • To engineering of non-leguminous plants for biosynthesis and accumulation of isoflavonoids, isolated isoflavone synthase (IFS) gene from a traditional medicinal plant Psoralea corylifolia and expressed in non-leguminous plants. The developed transgenic plants showed significant high accumulation of isoflavones in flower petals however no accumulation was observed id leaf tissue (Misra et al., Plant Cell Reports 2010a).
  • Transgenic tobacco and tomato plants developed using various transcription factors with increased biosynthesis and accumulation of the substrate flux for IFS as well as flavonols (Misra et al., Plant Physiology 2010b; Pandey et al., Plant Cell Reports 2012; Pandey et al., Scientific Reports 2014; Pandey et al., Scientific Reports 2015a; Pandey et al., Plant Cell Reports 2015b).
  • Global-gene expression and metabolome in different tissues of the transgenic lines of tobacco and tomato suggested role of AtMYB12 in regulation number of pathways, leading to flux availability for phenylpropanoid pathway in general and flavonol biosynthesis in particular (Misra et al., Plant Physiology 2010b; Pandey et al., Scientific Reports 2015a). Demonstrated involvement of light associated factors in regulation of flavonoid biosynthesis (Bhatia et al., Plant Cell Physiology, 2018).
  • The transgenic tobacco lines expressing AtMYB12 showed significant resistance against two major insect pests, S. litura and H. armigera due to enhanced accumulation of rutin and role of rutin for insect resistance has been well validated through artificial miRNA (Misra et al., Plant Physiology 2010b; Pandey et al., Plant Cell Reports 2012).
  • Transgenic tobacco plants developed with co-expression of AtMYB12 and GmIFS showed substantial accumulation of genistein glycoconjugates to the highest levels that could be engineered in tobacco leaves till date. Estrogen deficient (ovariectomized, Ovx) mice fed with leaf extract from the transgenic plant coexpressing AtMYB12 and GmIFS showed improved bone health (Pandey et al., Plant Biotechnology Journal 2014). Extracts from AtMYB12 expressing flavonol enriched tomato fruit led to the modulation in chondrogenesis to increase bone length in growing animals (Chaudhari et al., Scientific Reports 2016).
  • Identified and functionally characterized miRNA which targets MYB transcription factors involved in regulation of phenylpropanoid pathway. Analysis of miR858 overexpressing and mimic lines suggests involvement of this miRNA in plant growth and development and flavonoid biosynthesis (Sharma et al., Plant Physiology 2016).
  • Established transcriptome sequences of three distinct chemotypes of Withania somnifera (leaf and root) with distinct chemical profiles. Established correlation of transcriptome with metabolic profile and identified genes involved in biosynthesis of specific withanolides (Gupta et al., PLoS One 2013; Gupta et al., Scientific Reports 2015; Agrarwal et al., 2017). Developed Withania omics database which ready for the release. Validated involvement of number of genes in withanolide biosynthesis through Virus Induced Gene Silencing (VIGS) approach (Agarwal et al., Plant cell and Physiology, 2018).
  • Established a genetic transformation system for Withania somnifera and has characterized important genes involved in biosynthesis of withanolides (Pandey et al., Plant Cell Reports 2010; Gupta et al., Plant Growth Regulation 2011; Gupta et al., Protoplasma 2013; Akhtar et al., Protoplasma 2013; Agarwal et al., Functional and Integrative Genomics 2017).
  • Established transcriptome of poppy germplam lines BR086 and pap1 mutant and identified key genes and transcription factors putatively involved in papaverine biosynthesis as well as other alkaloids (Pathak et al. PLoS One 2013; Agarwal et al., Protoplasma 2016). Established high frequency somatic embryogenesis and regeneration systems (Pathak et al., Plant Growth Regulation 2012) as well as Virus Induced Gene Silencing (VIGS) protocol for functional genomics of poppy.
  • Established comprehensive transcriptomes datasets of neem (Azadirahta indica) and identified gene networks involved in azadirachtin biosynthesis pathway (Bhambani et al., Scientific Reports 2017).
  • Used CRISPR/Cas9 approach to establish involvement of small peptide (miPEP858) encoded by pri-miR858 and established regulatory role of miPEP in controlling expression of various genes and biological processes (Sharma et al., 2020, Nature Plants).

Environmental Biotechnology

  • Carried out extensive studies and suggested that phytochelatins (PC) and antioxidant systems play important role in detoxification of heavy metals in different plants (Srivastava et al., Environ Sci Technol 2007; Mishra et al., Aquat Toxicol 2008; Mishra et al., Bioresour Technol 2009; Shri et al., Ecotoxicology and Environmental Safety 2009; Dwivedi et al., Protoplasma 2010a; Dwivedi et al., J Hazard Mater 2010b; Singh et al., Bioresour Technol 2010a; Singh et al., Protoplasma 2010b; Tripathi et al., Environ Monit Assess 2012; Dwivedi et al., Environment International, 2012; Tripathi et al., Ecotoxicol Environ Saf. 2012; Tripathi et al., Ecological Engineering 2013; Tripathi et al., Environmental Science and Pollution Research 2013; Dave et al., Journal of Hazardous Materials 2013; Dixit et al., Plant Physiology and Biochemistry 2015).
  • PC synthase gene isolated from the aquatic plant, Ceratophyllum demersum and developed transgenic plants. Transgenic plants showed significant enhanced tolerance and accumulation to different heavy metals (Shukla et al., Plant Cell Reports 2012; Shukla et al., Protoplasma 2013a). Rice transgenic lines expression this PC gene showed significantly reduced arsenic content in grain suggesting that this gene can be used to develop low arsenic accumulating rice varieties (Shri et al., Scientific Reports 2014).
  • With transgenic approach, successfully demonstrated that synthetic phytochelatins can be used to enhance accumulation of heavy metal and loids for phytoremediation application (Shukla et al., Biochemical and Biophysical Research Communications 2013b).
  • The effect of different heavy metals on genome-wide expression has been studied in rice. Studies suggests that common as well different metabolic networks operate for detoxification of different heavy metals in plants (Chakrabarty et al., Chemosphere 2009; Tuli et al., Molecular Breeding 2010; Dubey et al., BMC Genomics 2010; Rai et al., Chemosphere 2011, Kumar et al., Functional and Integrative Genomics 2011; Kumar et al., Journal of Hazardous Materials 2013; Kumar et al., Plant Molecular Biology Reports 2013; Rai et al., Plant Genome 2015). Identified miRNAs involved in contrasting arsenic stress response in different rice cultivars (Sharma et al., Metallomics 2015).
  • Functionally characterized various genes and showed their involvement in heavy metal tolerance, accumulation and transport (Tiwari et al., Scientific Reports 2014; Tiwari et al., Plant Cell and Environment 2014; Kumar et al., Journal of Hazardous Materials 2013, Kumar et al., Plant Signaling and Behaviour 2015; Rai et al., PLoS One 2015).
  • Studied natural variation in Arabidopsis and suggested differential regulatory mechanism in various ecotypes under arsenic stress (Shukla et al., Frontiers in Plant Science, 2015; Khare et al., Journal of Hazardous Materials 2017; Shukla et al., Journal of Hazardous Materials 2018; Khare et al., Journal of Hazardous Materials 2019)

Molecular Basis of Fruit Ripening

  • Identified and characterized genes responsible for ethylene biosynthesis, perception as well as genes responsible for fruit quality in apple. The ACS1 and ERS1 genes were established to play regulatory role in apple fruit ripening (Asif et al., Journal of Agricultural and Food Chemistry 2006; Asif et al., South African Journal of Botany 2009).
  • During banana ripening, activities of the cell wall hydrolases PME, PG, cellulase and PL studied showed that ripening is largely dependent on ethylene production and in planta perception by banana fruit tissue (Lohani et al., Postharvest Biology and Technology 2004).
  • Different fruit-specific and ripening-related genes and promoters from banana isolated and characterized (Trivedi et al., Plant Science 2004; Kesari et al., Postharvest Biology and Technology 2007; Kesari et al., Postharvest Biology and Technology 2010).
  • Developed transgenic tomato and banana plants with delayed ripening (Batra et al., Current Science 2010).
  • Established transcriptomes of ripe and unripe banana fruit and identified gene networks involved in fruit ripening (Asif et al., BMC Plant Biology 2014). Identified members of different gene families as well as miRNAs which might be involved in banana fruit ripening (Asif et al., Functional and Integrative Genomics, 2014; Lakhwani et al., Scientific Reports 2015; Pandey et al., Scientific Reports 2016; Goel et al., Functional and Integrative Genomics 2016).

Major R&D Projects coordinated and Fund Mobilization in Specialized Areas as Nodal Scientist, PI, and Co-PI in last 10 years

  • Genome-editing for enhanced yield and quality traits (GE-plant) CSIR project under NCP category (PI and Coordinator)
  • Small RNAs and Associated factors for enhanced post-harvest Life (sRNA-life) CSIR project under FBR category (PI and Coordinator)
  • CRISPR-mediated genome editing of plant miRNAs for functional validation and biogenesis. Dept. of Biotechnology, Govt. of India (PI) Biotechnological Interventions through RNAi approach for management of Banana Bunchy Top Virus (BBTV) in Northeast region of India. Collaborative project under DBT’s NER-Banana Program for NE from Dept. of Biotechnology, Govt. of India (PI)
  • Biotechnological Interventions through RNAi approach for management of Banana Bunchy Top Virus (BBTV) in Northeast region of India. Collaborative project under DBT’s NER-Banana Program for NE from Dept. of Biotechnology, Govt. of India (PI)
  • Functional characterization of light-associated factor(s) regulating flavonoid biosynthesis in tomato. Project under TATA Innovation Fellowship from Dept. of Biotechnology, Govt. of India (PI)
  • Secondary plant product pathway engineering for enhanced nutritional quality and yield (Center of Excellent) Dept. of Biotechnology, Govt. of India (PI) 2017-2022 (PI)
  • CSIR - BMBF Cooperative Science Program (2015-17)
  • Genomics of Medicinal plants and Agronomically Important Traits (PlaGen) (2012-2017) I was the Nodal Scientist and coordinating this CSIR Network Project with seven different CSIR laboratories
  • Functional characterization of chromium induced glutathione-s-transferases from rice using heterologous system (2012-2015) Dept. of Science and Technology, Govt. of India (Co-PI)
  • Role of miRNAs in arsenic uptake and transport in rice. (2011-2014) Dept of Biotechnology, Govt. of India (PI)
  • Leads based drug development and genetic improvement of Ashwagandha (Withania somnifera)” (2010-2015) CSIR-NMITLI project (PI)
  • Synthetic biology and metabolic engineering of azadirachtin biosyntehsis pathway (2010-2012) CSIR Network project (HCP-02)
  • Functional analysis of sterol glycosyltransferase (SGT) genes of Withania somnifera using miRNA technology. Dept. of Science and Technology, Govt. of India (Co-PI)
  • Role of thiol and nitric oxide metabolism mediated pathways in arsenic stress in higher plants (2010-2013) Indo-Spanish joint project Dept. of Science and Technology, Govt. of India (Co-PI)
  • Metabolic Engineering of Vinca Alkaloid Pathway (2010-2012) CSIR Network project (HCP-03)
  • Functional analysis of members of sterol glycosyltransferase gene family of Withania somnifera using homologous and heterologous expression system (2010 to 2013) Dept of Biotechnology, Govt. of India (Co-PI)
  • Pathway engineering and system biology approach towards homologous and heterologous expression of high-value phytoceuticals (2007-2012) CSIR Network project (Nodal officer NBRI)
  • Development of transgenic rice plant and hyper accumulator plant for arsenic contaminated environment (2007-2012) CSIR Network project (Co-PI)
  • Environmental contaminants – New screening technologies and effect on human health (2007-2012) CSIR Network project (Co-PI)

Chapters in the books

  • Nath P, Sane VA, Sane AP, Trivedi PK (2005) Plant Gene Expression, Regulation of. In: Encyclopedia of Molecular and Cell Biology and Molecular Medicine. Ed. R. A. Meyer, Wiley-VCH Verlag Gmbh & Co, Weinheim, Germany. Vol 10 (2nd edition) pp 307-358.
  • Nath P, Sane AP, Trivedi PK, Sane VA (2005) Understanding gene expression during plant growth and development. In Golden Jubilee Volume, NBRI, Lucknow, pp 207-213.
  • Nath P, Trivedi PK, Sane VA, Sane AP (2006) Role of ethylene in fruit ripening. (ed. Khan NA) Springer-Verlog Berlin Heidelberg. pp 151-176.
  • Nath P, Sane AP, Trivedi PK, Sane VA, Asif M (2007) Role of transcription factors in regulating ripening, senescence and organ abscission in plants. Steward Postharvest Reviews pp 1-14.
  • Tuteja N, Gill SS, Trivedi PK, Asif MH, Nath P (2010) Plant Growth Regulators and their Role in Stress Tolerance. In: Anjum NA (Ed) Plant Nutrition and Abiotic Stress Tolerance I. Plant Stress 4 (Special Issue 1), 1-18
  • Tuli R, Tripathi RD, Chakrabarty D, Adhikari B, Trivedi PK (2010) Arsenic hazards associated with food security. SATSA Mukhapatra - Annual Technical Issue 14: 82-97.
  • Contributed in book entitled “Ashwagandha (Withania somnifera): A model Indian Medicinal Plant” Ed. Tuli R and Sangwan RS, Army Printing Press, Lucknow.
  • Trivedi, P. K., Akhtar, N., Gupta, P. and Nath, P. (2013) Metabolomic Approaches for Improving Crops Under Adverse Conditions, in Climate Change and Plant Abiotic Stress Tolerance (eds N. Tuteja and S. S. Gill), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527675265.ch28
  • Nath P, Sane VA, Asif MH, Sane AP, Trivedi PK (2012) Fruit Crops: Omic Approaches towards Elucidation of Abiotic Stress Tolerance. (Tuteja N, Gill SS, Tiburcio AF, Tuteja R eds.) Improving Crop Resistance to Abiotic Stress. Wiley-VCH, Weinheim. pp 1033-1048.
  • Shukla D, Trivedi PK, Nath P, Tuteja N (2015) Metallothioneins and Phytochelatins: role and perspectives in heavy metal(loid)s stress tolerance in crop plants. In (Tuteja N and Gill SS, eds) Climate Change and Abiotic Stress Tolerance, Wiley, Germany (In Press)
  • Kumar S, Trivedi PK (2016) Heavy metal stress signalling in plants. In (Ahmad P, eds) Plant Metal Interaction, Elsevier Inc. pp: 581-599.
  • Kumar S and Trivedi PK (2016) Transcriptome modulation in rice under abiotic stress. In (Azooz MM and Ahmad P, eds) Plant-Environment Interaction: Responses and Approaches to Mitigate Stress, John Wiley & Sons, Ltd. pp 70-83
  • Kumar S and Trivedi PK (2019) Genomics of Arsenic stress response in plants. In (Rajpal VR, Sehgal D, Kumar A Raina, S(eds.)] Genetic Enhancement of Crops for Tolerance to Abiotic Stress: Mechanisms and Approaches, Vol. I, Springer International Publishing pp. 231-248
  • Pathak S, Agarwal AV, Agarwal P, Trivedi PK (2019) Secondary Metabolite Pathways in Medicinal Plants: Approaches in Reconstruction and Analysis. In [(Singh S, Upadhyay S, Pandey A, Kumar S (eds)] Molecular Approaches in Plant Biology and Environmental Challenges: Energy, Environment, and Sustainability. Springer, Singapore pp 339-364
  • Kumar RS, Singh D, Bose SK, Trivedi PK (2020) Biodegradation of environmental pollutant through pathways engineering and genetically modified organisms approaches. In [Chowdhary P, Raj A, Verma D, Akhter y (eds)] Microorganisms for Sustainable Environment and Health. Elsevier, Amsterdam, Netherlands, pp 137-166. DOI: 10.1016/B978-0-12-819001-2.00007-3
  • Sharma A, Badola P, Trivedi PK (2020) CRISPR-Cas9 System for Agriculture crop Improvement. In [Upadhyay S (eds)] Genome Engineering for Crop Improvement. Wiley (In Press)

Book Edited/Editing

Genetic Engineering of Plants: Enhancing Production and Value Addition (Eds. Trivedi PK, Nath P and Bouzayen Mondher) John Willey & Sons Limited (Book being edited)